An impressive example is the “super bloom” in Death Valley Death National Park, when seeds that have survived dry and hot deserts for decades suddenly grow in the rain, followed by rare and spectacular desert blooms for several months. The seed maintains a fully developed embryo that only continues to grow in the right conditions. This can only happen for years or, in extreme cases, centuries later. the initial events of seed germination.
New insights about the initial events of seed germination, Plant seeds sometimes seem unspecified, but they have properties that are nothing more than superpowers. When dry, they can save their energy for years and suddenly release it to germinate in favorable environmental conditions.
Seed germination controlled by several plant hormones, which examined in detail. But, not much known about the processes that need to carried out for hormones to work.
Using the new fluorescence biosensor, the researchers observed energy metabolism and sulfur-based redox metabolism in living sperm cells.
The researchers found that when seeds came into contact with water, energy metabolism formed in a matter of minutes and “cells” of plant cells, known as mitochondria, activated their breathing. The researchers also discovered which molecular switches activated to release energy efficiently with redox thiol switches, which play a central role.
To determine whether redox switches are important for initial germination, the researchers used genetic methods to deactivate certain proteins and then compare the reactions of modified seeds with those not modified.
The researchers artificially aged the seeds in the laboratory, and they saw that the germinated seeds were far less active if they lacked the right protein.
The next step of the researchers concerns what called Nar. Redox proteom analysis, e.g. They examined each redox protein as a whole using biochemical methods.
For this purpose, they isolate the active mitochondria and freeze them so that they can examine this situation directly where the process takes place. The researchers then used mass spectrometry to identify some so-called cysteine peptides that are important for resource efficiency in energy metabolism.