The CRISPR gene drive better solves old technology problems, Gene drives use genetic engineering to create the desired mutations in many individuals, which are then propagated by mating in populations of less than 10 generations.
In theory, such a mechanism could be used to prevent transmission of malaria mosquitoes or possibly eradicate invasive species by affecting their reproductive capacity.
Although scientists have been able to prove the concept in the laboratory, they have found that wild populations all adapt to schemes and develop resistance.
And when genetic discs work, they are all or nothing without nuances, they are distributed to all individuals, which can be detrimental.
Toxin-Antidote-CRISPR gene drive system for regional population modification.
This method can also be applied to regional populations, limiting the spread to other populations that might experience undesirable effects.
These are two things which to some extent overcome the new pursuit that we have developed here.
In the classic gene drive, known as the home drive, offspring inherit one set of genes or one genome from the mother and the other from the father. If the offspring inherit genes with a drive from one parent and not from another, the parent will copy it into the genome without a drive.
Now this individual has this effort in both genomes and will pass it on to each child.
Hard drives are equipped with CRISPR-Cas9 gene editing technology so that when a CRISPR machine is copied into a new genome without a drive, it cuts into the chromosomes and put them in the new code.
But sometimes cells repair incisions and erase all letters from DNA.
In this case, the activator of the CRISPR gene can no longer find the genetic sequence that is recognized for cutting, which leads to resistance and stops the spread of genes.
Natural genetic variations, another source of changes in DNA sequences, can also create resistance because CRISPR gene discs must recognize short genetic sequences to cut. “We were among the first laboratories to show that this was a big problem,” the researchers said.
The document describes an increase in a new gene called TARE (Toxin Antidote Recessive Embryo) which targets genes that are important for bodily functions.
At the same time, an organism can survive with only one complete copy of this main gene. Instead of cutting and pasting DNA like a self-correct device, the TARE device only cuts genes from other parents and deactivates them.
Meanwhile, the engineered TARE drive gene has a DNA sequence that has been coded; The gene works, but it will not be recognized or interrupted in future generations. If the offspring inherit two disabled genes, these individuals do not survive and delete this copy from the population.
As life partners, more living offspring carry the TARE gene drive.
Only a few people with home media can spread this feature throughout the population. In contrast, the TARE device does not cut off the device and put it in the target gene. Instead, they destroy one copy of the gene targeted offspring.
Therefore this drive requires a higher frequency of people in the population to spread. Therefore, TARE devices are less likely to move from one population to another.
In laboratory experiments, when fruit flies with the TARE-controlled gene are released into the cells of wild fruit flies, all the flies in the cell are only TARE for six generations.
Researchers have shown that while TARE aspirations can develop in nature, especially in very large populations, resistance, but they believe it will take longer and develop at a much slower rate, researchers say.